Hotspot

rpoB-trnC-GCA

LSC · 27428-28662 · 1235 bp

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Pi 0.00904

MarkerSeek score 53.50

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1235
variable_sites: 33
pi: 0.00904
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	GTTCTTCCGTCAAGCTCTGA	CCAGATTTGAACCGGGGAAA	50.0	50.0	57.6	58.1	1400-1403 bp	1.0	77.8
rpoB-trnC-GCA_p2	AGTTCTTCCGTCAAGCTCTG	CCAGATTTGAACCGGGGAAA	50.0	50.0	57.6	58.1	1401-1404 bp	1.0	77.7
rpoB-trnC-GCA_p3	CTTCCGTCAAGCTCTGATCA	CCAGATTTGAACCGGGGAAA	50.0	50.0	57.3	58.1	1397-1400 bp	1.0	76.0
rpoB-trnC-GCA_p4	GTTCTTCCGTCAAGCTCTGA	AAAAGGATTTGCAGTCCCCC	50.0	50.0	57.6	58.4	1382-1385 bp	1.0	75.6
rpoB-trnC-GCA_p5	AGTTCTTCCGTCAAGCTCTG	AAAAGGATTTGCAGTCCCCC	50.0	50.0	57.6	58.4	1383-1386 bp	1.0	75.5