Hotspot

rps1

Genome · 80728-81345 · 618 bp

Back to Polyopes

Pi 0.0777

MarkerSeek score 79.70

Primer available yes

Label rps1

Feature Metadata

label_name: rps1
feature_type: gene
region: Genome
length_bp: 618
variable_sites: 72
pi: 0.0777
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps1_p1	CTTTCCTCGCAGTCACCTAC	TCCATTGCCTTAACCCCTTG	55.0	50.0	58.0	57.8	1294-1302 bp	1.0	86.1
rps1_p2	GCGGTTTATTTCTGTGGCAC	TCCATTGCCTTAACCCCTTG	50.0	50.0	58.0	57.8	1313-1321 bp	1.0	85.8
rps1_p3	GCGATATAGTTGCAGGCACT	TCCATTGCCTTAACCCCTTG	50.0	50.0	58.1	57.8	1038-1046 bp	1.0	85.1
rps1_p4	ACCCTTGCCTAGCAGAAGTA	TCCATTGCCTTAACCCCTTG	50.0	50.0	58.0	57.8	1340 bp	0.666667	65.3
rps1_p5	AGCCATAAGCCGGATTTTGT	TCCATTGCCTTAACCCCTTG	45.0	50.0	57.9	57.8	1594 bp	0.666667	64.7