Hotspot

petA

Genome · 147988-148962 · 975 bp

Back to Polyopes

Pi 0.0995

MarkerSeek score 79.20

Primer available yes

Label petA

Feature Metadata

label_name: petA
feature_type: gene
region: Genome
length_bp: 975
variable_sites: 98
pi: 0.0995
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA_p1	AGCAACCGTCATTCATCCTC	AGGGGCTATTCTTACACCTTC	50.0	47.6	58.0	56.8	1721-1841 bp	0.666667	60.7
petA_p2	ATCTCGAGAAAGCAACCGTC	AGGGGCTATTCTTACACCTTC	50.0	47.6	58.0	56.8	1851 bp	0.333333	35.6
petA_p3	ATCTCGAGAAAGCAACCGTC	GGGGCTATTCTTACACCTTCT	50.0	47.6	58.0	56.8	1850 bp	0.333333	35.6
petA_p4	AGCAACCGTCATTCATCCTC	GGGGCTATTCTTACACCTTCT	50.0	47.6	58.0	56.8	1840 bp	0.333333	35.4
petA_p5	ATCTCGAGAAAGCAACCGTC	AGGGGCTATTCTTACACCTTCT	50.0	45.5	58.0	58.3	1851 bp	0.333333	35.2