Hotspot

orf12

Genome · 191611-192960 · 1350 bp

Back to Polyopes

Pi 0.2

MarkerSeek score 79.70

Primer available yes

Label orf12

Feature Metadata

label_name: orf12
feature_type: gene
region: Genome
length_bp: 1350
variable_sites: 271
pi: 0.2
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf12_p1	AATAAGCATCTGTGGCCGAG	CTCTCACACGTGTTGGACAA	50.0	50.0	58.0	58.1	2356 bp	0.666667	70.2
orf12_p2	AATAAGCATCTGTGGCCGAG	GGTCAGCATTTTCTTGTGGC	50.0	50.0	58.0	57.9	2589 bp	0.666667	70.1
orf12_p3	ACTCATGTGCGATCCGTTTT	CTCTCACACGTGTTGGACAA	45.0	50.0	58.2	58.1	2320 bp	0.666667	69.0
orf12_p4	AATAAGCATCTGTGGCCGAG	ATTGGCACAGTTCTTGGGAG	50.0	50.0	58.0	58.1	2298 bp	0.333333	52.3
orf12_p5	AATAAGCATCTGTGGCCGAG	CACGTGTTGGACAACCATTG	50.0	50.0	58.0	57.9	2350 bp	0.333333	52.1