Hotspot

psaD-acsF

Genome · 138834-139668 · 835 bp

Back to Polyopes

Pi 0.271

MarkerSeek score 78.70

Primer available yes

Label psaD-acsF

Feature Metadata

label_name: psaD-acsF
feature_type: igs
region: Genome
length_bp: 835
variable_sites: 226
pi: 0.271
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 78.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaD-acsF_p1	GCAGCTCTTAACCATCCTCC	TGAGCACCGCATCTATCCTA	55.0	50.0	58.0	58.0	1408-1459 bp	0.666667	71.2
psaD-acsF_p2	AGTTGAGCTTGTCATGGCAA	TGAGCACCGCATCTATCCTA	45.0	50.0	58.0	58.0	2082-2134 bp	0.666667	71.1
psaD-acsF_p3	CTCCTGTGCTTCCACCAAAT	TGAGCACCGCATCTATCCTA	50.0	50.0	58.1	58.0	1392-1443 bp	0.666667	70.9
psaD-acsF_p4	ACCAACTCTACCACCTCTCC	TGAGCACCGCATCTATCCTA	55.0	50.0	58.1	58.0	1850-1902 bp	0.666667	70.8
psaD-acsF_p5	AGTTGAGCTTGTCATGGCAA	CCCTGTAATGGCTGAATGCT	45.0	50.0	58.0	57.9	2313-2365 bp	0.666667	70.6