Hotspot

orf65-ycf45

Genome · 134678-136161 · 1484 bp

Back to Polyopes

Pi 0.288

MarkerSeek score 69.10

Primer available yes

Label orf65-ycf45

Feature Metadata

label_name: orf65-ycf45
feature_type: igs
region: Genome
length_bp: 1484
variable_sites: 132
pi: 0.288
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.31
markerseek_score: 69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf65-ycf45_p1	TGCTTGACCACAATCCACAA	AATGCTTCTAGCTCTGTGCC	45.0	50.0	57.9	58.0	2832 bp	0.333333	52.5
orf65-ycf45_p2	TGCTTGACCACAATCCACAA	GGTACGAGCTGCTAATGCTT	45.0	50.0	57.9	58.1	2845 bp	0.333333	52.3
orf65-ycf45_p3	TGCTTGACCACAATCCACAA	CCATCAGGAAATCGAGCCTC	45.0	55.0	57.9	58.1	2328 bp	0.333333	51.8
orf65-ycf45_p4	TGCTTGACCACAATCCACAA	ATGGTACGAGCTGCTAATGC	45.0	50.0	57.9	58.1	2847 bp	0.333333	51.8
orf65-ycf45_p5	TGCTTGACCACAATCCACAA	TGCTTCTAGCTCTGTGCCTA	45.0	50.0	57.9	58.1	2830 bp	0.333333	51.6