Hotspot

ycf65-groEL

Genome · 73336-73953 · 618 bp

Back to Polyopes

Pi 0.124

MarkerSeek score 79.20

Primer available yes

Label ycf65-groEL

Feature Metadata

label_name: ycf65-groEL
feature_type: igs
region: Genome
length_bp: 618
variable_sites: 113
pi: 0.124
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf65-groEL_p1	CCACGTAATGATGCATGGGA	ATTGTTCCTGGTGGTGGTTC	50.0	50.0	58.0	58.0	1172-1173 bp	1.0	87.8
ycf65-groEL_p2	CCACGTAATGATGCATGGGA	GTTGATTTGCACAGATGGGC	50.0	50.0	58.0	58.0	1134 bp	0.666667	68.9
ycf65-groEL_p3	CCACGTAATGATGCATGGGA	GATTGTTCCTGGTGGTGGTT	50.0	50.0	58.0	58.0	1174 bp	0.666667	68.7
ycf65-groEL_p4	CCACGTAATGATGCATGGGA	CGGTGGTGTCGCTGTTATAA	50.0	50.0	58.0	58.0	1285 bp	0.666667	68.6
ycf65-groEL_p5	CCACGTAATGATGCATGGGA	CTTTGTTGTCGCCATTGCAT	50.0	45.0	58.0	57.9	1061 bp	0.666667	68.4