Hotspot

ompR-psbD

Genome · 68592-68996 · 405 bp

Back to Polyopes

Pi 0.116

MarkerSeek score 80.70

Primer available yes

Label ompR-psbD

Feature Metadata

label_name: ompR-psbD
feature_type: igs
region: Genome
length_bp: 405
variable_sites: 70
pi: 0.116
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ompR-psbD_p1	GTTGATGACGAGACCAGCAT	CACCAATTTGACACCATCGC	50.0	50.0	58.0	58.0	1426 bp	0.666667	68.2
ompR-psbD_p2	GTTGATGACGAGACCAGCAT	TCTGCTGCATCACCATCTTC	50.0	50.0	58.0	58.0	1790 bp	0.666667	68.0
ompR-psbD_p3	GTTGATGACGAGACCAGCAT	AAACCAGCTAGCCTGTCCTA	50.0	50.0	58.0	58.0	1605 bp	0.666667	67.9
ompR-psbD_p4	GTTGATGACGAGACCAGCAT	AACCAGCTAGCCTGTCCTAA	50.0	50.0	58.0	58.0	1604 bp	0.666667	67.9
ompR-psbD_p5	GTTGATGACGAGACCAGCAT	TGTCAAGAAGTTGCAGCCTT	50.0	45.0	58.0	57.9	1326 bp	0.666667	67.8