Hotspot

rpl21-rpl32

Genome · 47853-48730 · 878 bp

Back to Polyopes

Pi 0.0926

MarkerSeek score 81.80

Primer available yes

Label rpl21-rpl32

Feature Metadata

label_name: rpl21-rpl32
feature_type: igs
region: Genome
length_bp: 878
variable_sites: 121
pi: 0.0926
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl21-rpl32_p1	AACCCACATTTGCCTTCCAT	GCTGGCTCTTGGGCTTTATA	45.0	50.0	58.0	57.7	1351-1353 bp	1.0	86.3
rpl21-rpl32_p2	ACCCACATTTGCCTTCCATT	GCTGGCTCTTGGGCTTTATA	45.0	50.0	58.0	57.7	1350-1352 bp	1.0	86.3
rpl21-rpl32_p3	AACCCACATTTGCCTTCCAT	ACACCTGTTTTAGCAGCTGG	45.0	50.0	58.0	58.4	1366-1368 bp	1.0	86.0
rpl21-rpl32_p4	ACCCACATTTGCCTTCCATT	ACACCTGTTTTAGCAGCTGG	45.0	50.0	58.0	58.4	1365-1367 bp	1.0	86.0
rpl21-rpl32_p5	ATTTTCCTGGCTGAACCCAC	GCTGGCTCTTGGGCTTTATA	50.0	50.0	58.4	57.7	1364-1366 bp	1.0	83.6