Hotspot

gltB-rpoZ

Genome · 22115-22486 · 372 bp

Back to Polyopes

Pi 0.0914

MarkerSeek score 79.70

Primer available yes

Label gltB-rpoZ

Feature Metadata

label_name: gltB-rpoZ
feature_type: igs
region: Genome
length_bp: 372
variable_sites: 51
pi: 0.0914
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
gltB-rpoZ_p1	AACCAACACCACATGCATCT	TGGTTGAACAAGCTGTGGTT	45.0	45.0	58.0	58.2	1079-1102 bp	1.0	88.4
gltB-rpoZ_p2	AACCAACACCACATGCATCT	AGGACACTAGAGTCTCCAGC	45.0	55.0	58.0	57.9	1209 bp	0.666667	67.1
gltB-rpoZ_p3	AACCAACACCACATGCATCT	GGACACTAGAGTCTCCAGCA	45.0	55.0	58.0	58.2	1208 bp	0.666667	66.9
gltB-rpoZ_p4	AACCAACACCACATGCATCT	TTGTGTAAGCATTGCCACCT	45.0	45.0	58.0	58.0	1121 bp	0.333333	53.3
gltB-rpoZ_p5	TTATACCTGCCCCATCACCA	TTGTGTAAGCATTGCCACCT	50.0	45.0	58.1	58.0	1247 bp	0.333333	52.5