Hotspot

rpl32-trnL-UAG

SSC · 122230-122914 · 685 bp

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Pi 0.0785
MarkerSeek score 75.40
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
685
variable_sites
178
pi
0.0785
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
75.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AAGACCAAGGGGAGTCTCTC GTAAAAATAGCGCCGGATGC 55.0 50.0 58.1 58.0 1617-1623 bp 0.833333 73.9
rpl32-trnL-UAG_p2 AAGACCAAGGGGAGTCTCTC TAAAAATAGCGCCGGATGCT 55.0 45.0 58.1 58.0 1616-1622 bp 0.833333 73.8
rpl32-trnL-UAG_p3 AAGACCAAGGGGAGTCTCTC GAAGTAGCAAAGCCTTGGGT 55.0 50.0 58.1 58.1 1635-1641 bp 0.833333 73.4
rpl32-trnL-UAG_p4 GACCAAGGGGAGTCTCTCAT GTAAAAATAGCGCCGGATGC 55.0 50.0 58.1 58.0 1615-1621 bp 0.833333 73.3
rpl32-trnL-UAG_p5 GACCAAGGGGAGTCTCTCAT TAAAAATAGCGCCGGATGCT 55.0 45.0 58.1 58.0 1614-1620 bp 0.833333 73.2