Hotspot

psaJ-rpl33

LSC · 70781-71343 · 563 bp

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Pi 0.0515

MarkerSeek score 71.30

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 563
variable_sites: 96
pi: 0.0515
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	GGATGCGTTAACATTCCCCT	TCATAGCTTCGAGTGGGGAA	50.0	50.0	57.9	58.1	1039-1078 bp	0.833333	75.3
psaJ-rpl33_p2	GGATGCGTTAACATTCCCCT	TTCATAGCTTCGAGTGGGGA	50.0	50.0	57.9	58.1	1040-1079 bp	0.833333	75.3
psaJ-rpl33_p3	ACCCGTGACATTTTGTACCC	GCTTGGAACAGTAGGGACAG	50.0	55.0	58.1	57.9	1323-1342 bp	0.833333	72.8
psaJ-rpl33_p4	CCCGTGACATTTTGTACCCA	GCTTGGAACAGTAGGGACAG	50.0	55.0	58.1	57.9	1322-1341 bp	0.833333	72.8
psaJ-rpl33_p5	GGATGCGTTAACATTCCCCT	GCTTGGAACAGTAGGGACAG	50.0	55.0	57.9	57.9	769 bp	0.833333	72.6