Hotspot

ycf4-cemA

LSC · 63556-64416 · 861 bp

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Pi 0.0486
MarkerSeek score 73.10
Primer available yes
Label ycf4-cemA

Feature Metadata

label_name
ycf4-cemA
feature_type
igs
region
LSC
length_bp
861
variable_sites
139
pi
0.0486
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf4-cemA_p1 TATGGGATCGCAGGTCTCTT TGTGTCTCCGGGTATTCCTT 50.0 50.0 57.9 58.1 1444-1451 bp 0.833333 73.0
ycf4-cemA_p2 TTCTATGGGATCGCAGGTCT TGTGTCTCCGGGTATTCCTT 50.0 50.0 57.9 58.1 1447-1454 bp 0.833333 73.0
ycf4-cemA_p3 TGAATTGGCGATCAGAACGT TGTGTCTCCGGGTATTCCTT 45.0 50.0 57.9 58.1 1647-1654 bp 0.833333 72.9
ycf4-cemA_p4 AATTACCCCGGCAGATTCAC TGTGTCTCCGGGTATTCCTT 50.0 50.0 57.9 58.1 1806-1813 bp 0.666667 66.4
ycf4-cemA_p5 AATTACCCCGGCAGATTCAC TCCACCAGGGTAAACAGACT 50.0 50.0 57.9 57.9 1634-1641 bp 0.666667 65.9