Hotspot

trnT-GGU-psbD

LSC · 34261-35797 · 1537 bp

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Pi 0.0431

MarkerSeek score 72.50

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1537
variable_sites: 219
pi: 0.0431
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	TTTACCAAGGGCTATAGTCA	55.0	40.0	58.1	53.0	1357-1666 bp	1.0	52.0
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	TTTACCAAGGGCTATAGTCA	50.0	40.0	58.1	53.0	1360-1669 bp	1.0	52.0
trnT-GGU-psbD_p3	GGCGTAAGTCATCGGTTCAA	TTTACCAAGGGCTATAGTCA	50.0	40.0	58.3	53.0	1334-1643 bp	1.0	52.0
trnT-GGU-psbD_p4	GTGGTAGAGTAACGCCATGG	TTACCAAGGGCTATAGTCAT	55.0	40.0	58.1	52.8	1356-1665 bp	1.0	52.0
trnT-GGU-psbD_p5	TCAGTGGTAGAGTAACGCCA	TTACCAAGGGCTATAGTCAT	50.0	40.0	58.1	52.8	1359-1668 bp	1.0	52.0