Hotspot

rpoB-trnC-GCA

LSC · 27966-29314 · 1349 bp

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Pi 0.0452

MarkerSeek score 73.90

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1349
variable_sites: 197
pi: 0.0452
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 73.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	GCCCAGGTATTGTCGACATT	CGAATCAAGCGCGTATCAAC	50.0	50.0	58.0	58.0	1533-1554 bp	0.833333	75.2
rpoB-trnC-GCA_p2	CCAAATTAACCCCGCGGATA	CGAATCAAGCGCGTATCAAC	50.0	50.0	58.0	58.0	1754-1775 bp	0.833333	75.0
rpoB-trnC-GCA_p3	TCGACCCCATAGCTGATGAT	CGAATCAAGCGCGTATCAAC	50.0	50.0	58.0	58.0	2058-2079 bp	0.833333	74.8
rpoB-trnC-GCA_p4	TAGACTCGACCCCATAGCTG	CGAATCAAGCGCGTATCAAC	55.0	50.0	58.0	58.0	2063-2084 bp	0.833333	74.8
rpoB-trnC-GCA_p5	CGAGCCCATATCCTTGCTTT	CGAATCAAGCGCGTATCAAC	50.0	50.0	58.0	58.0	2002-2023 bp	0.833333	74.8