Hotspot

atpH-atpI

LSC · 15073-15748 · 676 bp

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Pi 0.0457

MarkerSeek score 73.60

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 676
variable_sites: 98
pi: 0.0457
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	CCTCGTATTTTCCCCTCTGC	GCTGCTCGAAATCCACAAAC	55.0	50.0	58.0	58.0	1384-1404 bp	0.833333	74.7
atpH-atpI_p2	TACCTTCTACAGCTTGGCCT	GCTGCTCGAAATCCACAAAC	50.0	50.0	58.0	58.0	1347-1367 bp	0.833333	74.7
atpH-atpI_p3	TTCCCCCAGTTTTCTCAACG	GCTGCTCGAAATCCACAAAC	50.0	50.0	58.0	58.0	1603-1623 bp	0.833333	74.6
atpH-atpI_p4	TTCCCCCAGTTTTCTCAACG	TATCGTCCTTGGGTTCCCTT	50.0	50.0	58.0	58.0	1501-1521 bp	0.833333	74.6
atpH-atpI_p5	TTCCCCCAGTTTTCTCAACG	CTTATTAAGCTCAGCCGCCA	50.0	50.0	58.0	58.0	1625-1645 bp	0.833333	74.4