Hotspot

trnS-GCU-trnG-UCC

LSC · 9191-10366 · 1176 bp

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Pi 0.0416

MarkerSeek score 72.60

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 1176
variable_sites: 157
pi: 0.0416
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	TCCACTCAGCCATCTCTCC	ACGAATCACACTTTTACCACT	57.9	38.1	58.1	55.5	766-1281 bp	1.0	55.0
trnS-GCU-trnG-UCC_p2	TCCACTCAGCCATCTCTCC	AGAACGAATCACACTTTTACCAC	57.9	39.1	58.1	57.3	769-1284 bp	1.0	53.9
trnS-GCU-trnG-UCC_p3	TCCACTCAGCCATCTCTCC	GAACGAATCACACTTTTACCACT	57.9	39.1	58.1	57.3	768-1283 bp	1.0	53.9
trnS-GCU-trnG-UCC_p4	TCCACTCAGCCATCTCTCC	AACGAATCACACTTTTACCACT	57.9	36.4	58.1	56.2	767-1282 bp	1.0	53.1
trnS-GCU-trnG-UCC_p5	GTCCACTCAGCCATCTCTCC	ACGAATCACACTTTTACCACT	60.0	38.1	59.5	55.5	767-1282 bp	1.0	52.0