Hotspot

trnH-GUG-psbA

LSC · 77-412 · 336 bp

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Pi 0.0929

MarkerSeek score 63.50

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 336
variable_sites: 103
pi: 0.0929
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	CTGTTGCGTATTCAGCTCCT	55.0	50.0	58.1	58.0	948-1003 bp	1.0	90.8
trnH-GUG-psbA_p2	GATCCACTTGGCTACATCCG	ATTTCAGGCTGAGCACAACA	55.0	45.0	58.1	58.0	864-879 bp	0.833333	72.5
trnH-GUG-psbA_p3	GATCCACTTGGCTACATCCG	TTTCAGGCTGAGCACAACAT	55.0	45.0	58.1	58.0	863-878 bp	0.833333	72.5
trnH-GUG-psbA_p4	CAATCCACTGCCTTGATCCA	ATTTCAGGCTGAGCACAACA	50.0	45.0	57.9	58.0	878-893 bp	0.833333	72.4
trnH-GUG-psbA_p5	CAATCCACTGCCTTGATCCA	TTTCAGGCTGAGCACAACAT	50.0	45.0	57.9	58.0	877-892 bp	0.833333	72.4