Hotspot

ndhD-psaC

SSC · 125842-125972 · 131 bp

Back to Polygala

Pi 0.0504
MarkerSeek score 75.30
Primer available yes
Label ndhD-psaC

Feature Metadata

label_name
ndhD-psaC
feature_type
igs
region
SSC
length_bp
131
variable_sites
16
pi
0.0504
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.96
markerseek_score
75.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhD-psaC_p1 TGGAAAAACAACAATTATGGTTAACC AAACAACTCGAAGCATGGGT 30.8 45.0 57.3 58.0 198-205 bp 1.0 49.1
ndhD-psaC_p2 TGGAAAAACAACAATTATGGT AAACAACTCGAAGCATGGGT 28.6 45.0 52.1 58.0 198-205 bp 1.0 49.1
ndhD-psaC_p3 TGGAAAAACAACAATTATGGTTAACC AGATGTGAATCCGCTTGTCC 30.8 50.0 57.3 58.0 256-263 bp 1.0 47.1
ndhD-psaC_p4 TGGAAAAACAACAATTATGGTTAACC ATGTGAATCCGCTTGTCCAA 30.8 45.0 57.3 57.8 254-261 bp 1.0 47.1
ndhD-psaC_p5 TGGAAAAACAACAATTATGGTTAACC GATGTGAATCCGCTTGTCCA 30.8 50.0 57.3 58.3 255-262 bp 1.0 47.1