Hotspot

ndhF-rpl32

SSC · 121985-122440 · 456 bp

Back to Polygala

Pi 0.0751
MarkerSeek score 81.70
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
456
variable_sites
82
pi
0.0751
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.97
markerseek_score
81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 ATATGGGAACGGGAAGTGGA TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.1 57.9 1023-1290 bp 0.857143 82.8
ndhF-rpl32_p2 ATATGGGAACGGGAAGTGGA CAGCGTGTCTACCAGTTTCA 50.0 50.0 58.1 57.9 1014-1281 bp 0.857143 82.7
ndhF-rpl32_p3 ATATGGGAACGGGAAGTGGA AGCGTGTCTACCAGTTTCAC 50.0 50.0 58.1 57.8 1013-1280 bp 0.857143 82.7
ndhF-rpl32_p4 CATGAAGAGGGAATTGGGCA TCCTAAGAGCAGCGTGTCTA 50.0 50.0 57.9 57.9 1704-1971 bp 0.857143 81.0
ndhF-rpl32_p5 ACATGAAGAGGGAATTGGGC TCCTAAGAGCAGCGTGTCTA 50.0 50.0 57.9 57.9 1705-1972 bp 0.857143 80.9