Hotspot

ndhI-ndhF

IRb · 119519-119743 · 225 bp

Back to Polygala

Pi 0.0786

MarkerSeek score 76.20

Primer available yes

Label ndhI-ndhF

Feature Metadata

label_name: ndhI-ndhF
feature_type: igs
region: IRb
length_bp: 225
variable_sites: 41
pi: 0.0786
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-ndhF_p1	AGATGGACTTTACCCAGGCT	GGGGGTGGTCGAATTTCTTC	50.0	55.0	58.0	58.5	995-1031 bp	0.714286	70.7
ndhI-ndhF_p2	TTGCTTTGGGACGTTTACCA	GGGTGGTCGAATTTCTTCCT	45.0	50.0	57.9	57.5	378-413 bp	0.571429	69.4
ndhI-ndhF_p3	TTGCTTTGGGACGTTTACCA	GGGGTGGTCGAATTTCTTCC	45.0	55.0	57.9	58.5	379-414 bp	0.571429	69.0
ndhI-ndhF_p4	AGATGGACTTTACCCAGGCT	GGGTGGTCGAATTTCTTCCT	50.0	50.0	58.0	57.5	993-1029 bp	0.571429	64.2
ndhI-ndhF_p5	AGATGGACTTTACCCAGGCT	GGGGTGGTCGAATTTCTTCC	50.0	55.0	58.0	58.5	994-1030 bp	0.571429	63.8