Hotspot

infA-rps8

LSC · 79117-79244 · 128 bp

Back to Polygala

Pi 0.0767

MarkerSeek score 78.40

Primer available yes

Label infA-rps8

Feature Metadata

label_name: infA-rps8
feature_type: igs
region: LSC
length_bp: 128
variable_sites: 23
pi: 0.0767
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA-rps8_p1	GCCTACGGATTAAGCGACAT	CATGGGTAAGGACACTGTCG	50.0	55.0	57.8	58.0	844-980 bp	1.0	87.3
infA-rps8_p2	CTATAATCCGACCCCGCCTA	CATGGGTAAGGACACTGTCG	55.0	55.0	58.2	58.0	859-995 bp	1.0	87.3
infA-rps8_p3	CGAACTCGGAGAATAGCGTT	CATGGGTAAGGACACTGTCG	50.0	55.0	57.8	58.0	610-626 bp	1.0	85.2
infA-rps8_p4	GCGAGCCCCTTTCTCTTTTA	CATGGGTAAGGACACTGTCG	50.0	55.0	57.9	58.0	1052 bp	0.142857	44.8
infA-rps8_p5	GCGAGCCCCTTTCTCTTTTA	TCGATCAGAAGGAATTGGCG	50.0	50.0	57.9	58.1	692 bp	0.142857	44.4