Hotspot

psbJ-petA

LSC · 58707-59725 · 1019 bp

Back to Polygala

Pi 0.0307

MarkerSeek score 74.30

Primer available yes

Label psbJ-petA

Feature Metadata

label_name: psbJ-petA
feature_type: igs
region: LSC
length_bp: 1019
variable_sites: 79
pi: 0.0307
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbJ-petA_p1	TGGCTCATATTCTGGATTGGG	TGAGAAGGTTCAATTGTTCGA	47.6	38.1	57.5	55.5	1077-1104 bp	1.0	47.9
psbJ-petA_p2	GGCTCATATTCTGGATTGGGT	TGAGAAGGTTCAATTGTTCGA	47.6	38.1	57.5	55.5	1076-1103 bp	1.0	47.9
psbJ-petA_p3	TGGCTCATATTCTGGATTGGG	TGAGAAGGTTCAATTGTTCGAA	47.6	36.4	57.5	56.2	1077-1104 bp	1.0	47.9
psbJ-petA_p4	TGGCTCATATTCTGGATTGGG	TTGAGAAGGTTCAATTGTTCGA	47.6	36.4	57.5	56.2	1078-1105 bp	1.0	47.9
psbJ-petA_p5	GGCTCATATTCTGGATTGGGT	TGAGAAGGTTCAATTGTTCGAA	47.6	36.4	57.5	56.2	1076-1103 bp	1.0	47.9