Hotspot

atpH-atpI

LSC · 22923-24293 · 1371 bp

Back to Polygala

Pi 0.0384

MarkerSeek score 85.60

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1371
variable_sites: 130
pi: 0.0384
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 85.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	AAGCGGCATTCAAGCTCTTA	45.0	45.0	58.0	57.9	1470-1500 bp	1.0	88.8
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.0	57.9	1449-1479 bp	1.0	88.8
atpH-atpI_p3	AGCGGCAGAAATCAATGGAT	AAGCGGCATTCAAGCTCTTA	45.0	45.0	57.9	57.9	1462-1492 bp	1.0	88.7
atpH-atpI_p4	AGCGGCAGAAATCAATGGAT	TTTTGCAACTTTAGCTGCGG	45.0	45.0	57.9	57.9	1441-1471 bp	1.0	88.7
atpH-atpI_p5	ATAACGGAAGCGGCAGAAAT	CGAATCCATGGAGGGTCATC	45.0	55.0	58.0	57.8	1419-1449 bp	1.0	88.7