Hotspot

atpF-atpH

LSC · 22235-22676 · 442 bp

Back to Polygala

Pi 0.0395

MarkerSeek score 79.90

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 442
variable_sites: 48
pi: 0.0395
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	GGTGGCCAATAACCCAAGAA	GTTGTGGCATTAGCGCTTTT	50.0	45.0	58.1	57.9	511-548 bp	1.0	90.1
atpF-atpH_p2	GGTGGCCAATAACCCAAGAA	GTTGTGGCATTAGCGCTTTTA	50.0	42.9	58.1	57.8	511-548 bp	1.0	81.0
atpF-atpH_p3	GGCCAATAACCCAAGAAAAGG	GTTGTGGCATTAGCGCTTTT	47.6	45.0	57.4	57.9	508-545 bp	1.0	77.8
atpF-atpH_p4	GGTGGCCAATAACCCAAGAA	TTGTGGCATTAGCGCTTTTA	50.0	40.0	58.1	56.3	510-547 bp	1.0	77.3
atpF-atpH_p5	GGTGGCCAATAACCCAAGA	GTTGTGGCATTAGCGCTTTT	52.6	45.0	57.3	57.9	511-548 bp	1.0	77.2