Hotspot

trnG-UCC-trnR-UCU

LSC · 18603-18827 · 225 bp

Back to Polygala

Pi 0.0453
MarkerSeek score 81.40
Primer available yes
Label trnG-UCC-trnR-UCU

Feature Metadata

label_name
trnG-UCC-trnR-UCU
feature_type
igs
region
LSC
length_bp
225
variable_sites
29
pi
0.0453
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnR-UCU_p1 AGCCTTCCAAGCTAACGATG AGGTTTAGAAGACCTATGTCCT 50.0 40.9 58.0 55.6 303-315 bp 1.0 55.8
trnG-UCC-trnR-UCU_p2 AGCCTTCCAAGCTAACGATG AGAAGACCTATGTCCTATCCA 50.0 42.9 58.0 54.6 297-309 bp 1.0 55.8
trnG-UCC-trnR-UCU_p3 CCTAGCCTTCCAAGCTAACG AGGTTTAGAAGACCTATGTCCT 55.0 40.9 58.1 55.6 306-318 bp 1.0 55.5
trnG-UCC-trnR-UCU_p4 CCTAGCCTTCCAAGCTAACG AGAAGACCTATGTCCTATCCA 55.0 42.9 58.1 54.6 300-312 bp 1.0 55.4
trnG-UCC-trnR-UCU_p5 AGCCTTCCAAGCTAACGATG ACCAAAGGTTTAGAAGACCTATGT 50.0 37.5 58.0 57.6 308-320 bp 1.0 55.0