Hotspot

trnS-GCU-trnG-UCC

LSC · 16932-17789 · 858 bp

Back to Polygala

Pi 0.0435
MarkerSeek score 82.30
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
858
variable_sites
92
pi
0.0435
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 GCTTTAGTCCACTCAGCCAT AGGAAGGAGGACCCCTTAAC 50.0 55.0 57.6 58.0 755-1134 bp 1.0 87.9
trnS-GCU-trnG-UCC_p2 GCTTTAGTCCACTCAGCCAT GGAAGGAGGACCCCTTAACT 50.0 55.0 57.6 58.0 754-1133 bp 1.0 87.9
trnS-GCU-trnG-UCC_p3 AGTCCACTCAGCCATCTCTC AGGAAGGAGGACCCCTTAAC 55.0 55.0 58.5 58.0 750-1129 bp 1.0 87.1
trnS-GCU-trnG-UCC_p4 AGTCCACTCAGCCATCTCTC GGAAGGAGGACCCCTTAACT 55.0 55.0 58.5 58.0 749-1128 bp 1.0 87.1
trnS-GCU-trnG-UCC_p5 TAGTCCACTCAGCCATCTCT GGAAGGAGGACCCCTTAACT 50.0 55.0 57.2 58.0 750-1129 bp 1.0 84.5