Hotspot

trnL-UAA-trnQ-UUG

LSC · 14572-15272 · 701 bp

Back to Polygala

Pi 0.0432

MarkerSeek score 80.50

Primer available yes

Label trnL-UAA-trnQ-UUG

Feature Metadata

label_name: trnL-UAA-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 701
variable_sites: 74
pi: 0.0432
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnQ-UUG_p1	ACCAATTTCGCCATATCCCC	TTTTGGTCCCGCTATTCGAG	50.0	50.0	58.0	58.0	743-763 bp	0.857143	83.8
trnL-UAA-trnQ-UUG_p2	TAGCGTCTACCAATTTCGCC	TTTTGGTCCCGCTATTCGAG	50.0	50.0	58.1	58.0	751-771 bp	0.857143	83.2
trnL-UAA-trnQ-UUG_p3	ACCAATTTCGCCATATCCCC	GTTTTGGTCCCGCTATTCGA	50.0	50.0	58.0	58.3	744-764 bp	0.857143	81.8
trnL-UAA-trnQ-UUG_p4	AGTCCGTAGCGTCTACCAAT	TTTTGGTCCCGCTATTCGAG	50.0	50.0	58.2	58.0	757-777 bp	0.857143	81.7
trnL-UAA-trnQ-UUG_p5	TAGCGTCTACCAATTTCGCC	GTTTTGGTCCCGCTATTCGA	50.0	50.0	58.1	58.3	752-772 bp	0.857143	81.1