Hotspot

trnI-AAU-ndhC

LSC · 10591-11189 · 599 bp

Back to Polygala

Pi 0.065

MarkerSeek score 77.10

Primer available yes

Label trnI-AAU-ndhC

Feature Metadata

label_name: trnI-AAU-ndhC
feature_type: igs
region: LSC
length_bp: 599
variable_sites: 87
pi: 0.065
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-AAU-ndhC_p1	GGTTCGAATCCGTATAGCCC	TGCCCAAAGAATATCATATTCGT	55.0	34.8	57.9	56.1	563-657 bp	1.0	52.3
trnI-AAU-ndhC_p2	CGGTTCGAATCCGTATAGCC	TGCCCAAAGAATATCATATTCGT	55.0	34.8	58.3	56.1	564-658 bp	1.0	52.0
trnI-AAU-ndhC_p3	GGTTCGAATCCGTATAGCCC	TCATATTCGTAAAGCAAAAGCA	55.0	31.8	57.9	54.7	550-644 bp	1.0	52.0
trnI-AAU-ndhC_p4	GGTTCGAATCCGTATAGCCC	ATGCCCAAAGAATATCATATTCGT	55.0	33.3	57.9	56.6	564-658 bp	1.0	52.0
trnI-AAU-ndhC_p5	CGGTTCGAATCCGTATAGCC	TCATATTCGTAAAGCAAAAGCA	55.0	31.8	58.3	54.7	551-645 bp	1.0	52.0