Hotspot

ndhD-psaC

SSC · 119406-119547 · 142 bp

Back to Poilannammia

Pi 0.0496

MarkerSeek score 71.90

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 142
variable_sites: 7
pi: 0.0496
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	ACCCGCTAATATTGGTAAACCT	AAACAACTCGAAGCATGGGT	40.9	45.0	57.2	58.0	227-228 bp	1.0	60.4
ndhD-psaC_p2	ACCCGCTAATATTGGTAAACCT	GGCATGAAACAACTCGAAGC	40.9	50.0	57.2	58.0	233-234 bp	1.0	60.2
ndhD-psaC_p3	AACCCGCTAATATTGGTAAACCT	AAACAACTCGAAGCATGGGT	39.1	45.0	57.9	58.0	228-229 bp	1.0	57.7
ndhD-psaC_p4	AACCCGCTAATATTGGTAAACCT	GGCATGAAACAACTCGAAGC	39.1	50.0	57.9	58.0	234-235 bp	1.0	57.5
ndhD-psaC_p5	ACCCGCTAATATTGGTAAACCT	AGAGATGTGAATCCGCTTGC	40.9	50.0	57.2	58.3	287-288 bp	1.0	57.2