Hotspot

ndhF-rpl32

SSC · 115025-116001 · 977 bp

Back to Poilannammia

Pi 0.0208

MarkerSeek score 61.40

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 977
variable_sites: 20
pi: 0.0208
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGGGAAGTGGAATGAGAGGT	TGAAAAAGCCTTCAATGCTGT	50.0	38.1	58.0	56.9	1128-1150 bp	1.0	65.4
ndhF-rpl32_p2	TGGGAAGTGGAATGAGAGGT	ATGAAAAAGCCTTCAATGCTGT	50.0	36.4	58.0	57.4	1129-1151 bp	1.0	61.3
ndhF-rpl32_p3	TGGGAAGTGGAATGAGAGGT	TCGCTAATGAAAAAGCCTTCA	50.0	38.1	58.0	56.2	1135-1157 bp	1.0	59.5
ndhF-rpl32_p4	TGGGAAGTGGAATGAGAGGT	TGAAAAAGCCTTCAATGCTG	50.0	40.0	58.0	55.1	1128-1150 bp	1.0	58.6
ndhF-rpl32_p5	TGGGAAGTGGAATGAGAGGT	GAAAAAGCCTTCAATGCTGT	50.0	40.0	58.0	55.0	1127-1149 bp	1.0	58.6