Hotspot

psbT-pbf1

LSC · 75967-76038 · 72 bp

Back to Poilannammia

Pi 0.141

MarkerSeek score 88.80

Primer available yes

Label psbT-pbf1

Feature Metadata

label_name: psbT-pbf1
feature_type: igs
region: LSC
length_bp: 72
variable_sites: 10
pi: 0.141
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 88.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-pbf1_p1	CCGCCGAAAGTTCCAACTAA	ATCCATTCGAAGAACACGGG	50.0	50.0	58.5	58.0	130-131 bp	1.0	82.7
psbT-pbf1_p2	CCGCCGAAAGTTCCAACTAA	TTCGAAGAACACGGGGACTA	50.0	50.0	58.5	58.4	125-126 bp	1.0	80.1
psbT-pbf1_p3	TTTTTCGAGAACCGCCGAA	ATCCATTCGAAGAACACGGG	47.4	50.0	58.0	58.0	141-142 bp	1.0	77.9
psbT-pbf1_p4	CCGCCGAAAGTTCCAACTA	ATCCATTCGAAGAACACGGG	52.6	50.0	57.8	58.0	130-131 bp	1.0	76.8
psbT-pbf1_p5	CCGCCGAAAGTTCCAACTAA	TCGAAGAACACGGGGACTAG	50.0	55.0	58.5	58.8	124-125 bp	1.0	76.6