Hotspot

psbB-psbT

LSC · 75673-75858 · 186 bp

Back to Poilannammia

Pi 0

MarkerSeek score 29.50

Primer available yes

Label psbB-psbT

Feature Metadata

label_name: psbB-psbT
feature_type: igs
region: LSC
length_bp: 186
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 29.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-psbT_p1	GCTGGTATTGACCCGGATTT	TTAGTTGGAACTTTCGGCGG	50.0	50.0	57.9	58.5	371 bp	1.0	75.7
psbB-psbT_p2	CCAACGACAAGAAGACAGGT	TTAGTTGGAACTTTCGGCGG	50.0	50.0	57.8	58.5	308 bp	1.0	74.2
psbB-psbT_p3	CCGGATTTGGATGCTCAAGT	TTAGTTGGAACTTTCGGCGG	50.0	50.0	58.2	58.5	359 bp	1.0	74.2
psbB-psbT_p4	TGGTATTGACCCGGATTTGG	TTAGTTGGAACTTTCGGCGG	50.0	50.0	57.6	58.5	369 bp	1.0	72.7
psbB-psbT_p5	TTTTGCTGGTATTGACCCGG	TTAGTTGGAACTTTCGGCGG	50.0	50.0	58.5	58.5	375 bp	1.0	72.4