Hotspot

trnW-CCA-trnP-UGG

LSC · 67633-67850 · 218 bp

Back to Poilannammia

Pi 0.0157

MarkerSeek score 64.10

Primer available yes

Label trnW-CCA-trnP-UGG

Feature Metadata

label_name: trnW-CCA-trnP-UGG
feature_type: igs
region: LSC
length_bp: 218
variable_sites: 3
pi: 0.0157
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnW-CCA-trnP-UGG_p1	GTTTTGGAGACCCACGTTCT	AAGAAAGGGATGTAGCGCAG	50.0	50.0	58.0	58.0	311-338 bp	1.0	84.2
trnW-CCA-trnP-UGG_p2	TTTTGGAGACCCACGTTCTG	AAGAAAGGGATGTAGCGCAG	50.0	50.0	58.0	58.0	310-337 bp	1.0	84.2
trnW-CCA-trnP-UGG_p3	TGCCGAACTGAACTAAGAGC	AAGAAAGGGATGTAGCGCAG	50.0	50.0	57.9	58.0	292-319 bp	1.0	83.9
trnW-CCA-trnP-UGG_p4	ACCTACGACATCGGGTTTTG	AAGAAAGGGATGTAGCGCAG	50.0	50.0	57.9	58.0	325-352 bp	1.0	83.8
trnW-CCA-trnP-UGG_p5	GTTTTGGAGACCCACGTTCT	GGGTACAAAATGTCACGGGT	50.0	50.0	58.0	58.1	271-298 bp	1.0	83.8