Hotspot

psbZ-trnG-UCC

LSC · 37145-37457 · 313 bp

Back to Poilannammia

Pi 0.0192

MarkerSeek score 63.80

Primer available yes

Label psbZ-trnG-UCC

Feature Metadata

label_name: psbZ-trnG-UCC
feature_type: igs
region: LSC
length_bp: 313
variable_sites: 6
pi: 0.0192
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-UCC_p1	GACGGCTGGTCAAGTAACAA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.1	57.8	459 bp	1.0	78.7
psbZ-trnG-UCC_p2	CCCGTTGTTTTTGCTTCTCC	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.9	57.8	480 bp	1.0	78.6
psbZ-trnG-UCC_p3	ACGGCTGGTCAAGTAACAAA	CGTCTTCTCCTTGGCAAAGA	45.0	50.0	57.7	57.8	458 bp	1.0	77.0
psbZ-trnG-UCC_p4	GTTTTTGCTTCTCCTGACGG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.3	57.8	474 bp	1.0	74.1
psbZ-trnG-UCC_p5	GACGGCTGGTCAAGTAACAA	AACTTAACTCGGGCGGATAG	50.0	50.0	58.1	57.1	494 bp	1.0	72.9