Hotspot

atpH-atpI

LSC · 13884-14838 · 955 bp

Back to Poilannammia

Pi 0.0105

MarkerSeek score 65.10

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 955
variable_sites: 10
pi: 0.0105
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	GAAGCAAGTCCGACAGCTAA	TAGCCGCCGCTTATATAGGA	50.0	50.0	57.9	57.9	1059-1061 bp	1.0	79.3
atpH-atpI_p2	GAAGCAAGTCCGACAGCTAA	TTTTTGCAACTTTAGCCGCC	50.0	45.0	57.9	58.1	1071-1073 bp	1.0	79.2
atpH-atpI_p3	AACAGAAGCGGCAGAAATCA	TAGCCGCCGCTTATATAGGA	45.0	50.0	58.1	57.9	1027-1029 bp	1.0	79.1
atpH-atpI_p4	AACAGAAGCGGCAGAAATCA	TTTTTGCAACTTTAGCCGCC	45.0	45.0	58.1	58.1	1039-1041 bp	1.0	79.0
atpH-atpI_p5	CAGCAGCAATAACAGAAGCG	TAGCCGCCGCTTATATAGGA	50.0	50.0	57.8	57.9	1037-1039 bp	1.0	78.6