Hotspot

accD

LSC · 57196-58689 · 1494 bp

Back to Poikilospermum

Pi 0.00201

MarkerSeek score 59.90

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1494
variable_sites: 3
pi: 0.00201
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	TCATGGAAATTAGGGGCAAGA	AAATAACTGCTTGGTCGCCT	42.9	45.0	57.0	57.8	1602 bp	1.0	62.2
accD_p2	TCATGGAAATTAGGGGCAAGA	AATAACTGCTTGGTCGCCTC	42.9	50.0	57.0	58.3	1601 bp	1.0	61.7
accD_p3	TGGAAATTAGGGGCAAGAAAGT	AAATAACTGCTTGGTCGCCT	40.9	45.0	57.9	57.8	1599 bp	1.0	61.5
accD_p4	TCATGGAAATTAGGGGCAAGA	TTGGTCGCCTCCAAACAAAT	42.9	45.0	57.0	58.3	1592 bp	1.0	61.4
accD_p5	TGGAAATTAGGGGCAAGAAAGT	AATAACTGCTTGGTCGCCTC	40.9	50.0	57.9	58.3	1598 bp	1.0	61.1