Hotspot

ycf1-trnN-GUU

IRa · 129500-129812 · 313 bp

Back to Poikilospermum

Pi 0

MarkerSeek score 25.80

Primer available yes

Label ycf1-trnN-GUU

Feature Metadata

label_name: ycf1-trnN-GUU
feature_type: igs
region: IRa
length_bp: 313
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 25.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-trnN-GUU_p1	ATAGAGTCCGACCACAACGA	ACGACCAGTCAGTTAACAGC	50.0	50.0	58.2	57.8	446 bp	1.0	77.4
ycf1-trnN-GUU_p2	ATAGAGTCCGACCACAACGA	AGGATTCGAACCTACGACCA	50.0	50.0	58.2	58.2	459 bp	1.0	77.4
ycf1-trnN-GUU_p3	ATAGAGTCCGACCACAACGA	GGATTCGAACCTACGACCAG	50.0	55.0	58.2	57.8	458 bp	1.0	77.0
ycf1-trnN-GUU_p4	ATAGAGTCCGACCACAACGA	CCCCAAGTAGGATTCGAACC	50.0	55.0	58.2	57.7	467 bp	1.0	76.0
ycf1-trnN-GUU_p5	ATAGAGTCCGACCACAACGA	TTCGAACCTACGACCAGTCA	50.0	50.0	58.2	58.4	455 bp	1.0	75.5