Hotspot

rpl32-trnL-UAG

SSC · 113239-114095 · 857 bp

Back to Poikilospermum

Pi 0.00476

MarkerSeek score 63.30

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 857
variable_sites: 4
pi: 0.00476
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGGCAGCATTGAAAGCTTTT	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.8	58.0	956-969 bp	1.0	80.2
rpl32-trnL-UAG_p2	GGGCAGCATTGAAAGCTTTT	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.8	58.0	957-970 bp	1.0	80.2
rpl32-trnL-UAG_p3	GGGCAGCATTGAAAGCTTTT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	57.8	57.9	966-979 bp	1.0	79.3
rpl32-trnL-UAG_p4	GGGCAGCATTGAAAGCTTTT	TAGAAAATGACATGCCGCCA	45.0	45.0	57.8	57.9	1019-1032 bp	1.0	79.2
rpl32-trnL-UAG_p5	GGGCAGCATTGAAAGCTTTT	CTAAGAGCAGCGTGTCTACC	45.0	55.0	57.8	57.8	964-977 bp	1.0	78.7