Hotspot

trnR-ACG-trnN-GUU

IRb · 107779-108353 · 575 bp

Back to Poikilospermum

Pi 0.00696

MarkerSeek score 62.20

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: IRb
length_bp: 575
variable_sites: 4
pi: 0.00696
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	CAGAGGATTAGAGCACGTGG	TGGGTTGAGAACGGGAATTG	55.0	50.0	57.8	58.1	757 bp	1.0	77.9
trnR-ACG-trnN-GUU_p2	CAGAGGATTAGAGCACGTGG	GCTGTTAACTGACTGGTCGT	55.0	50.0	57.8	57.8	680 bp	1.0	77.5
trnR-ACG-trnN-GUU_p3	CAGAGGATTAGAGCACGTGG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	57.8	58.2	667 bp	1.0	77.5
trnR-ACG-trnN-GUU_p4	GAACAGATCCAGTGGAGACG	TGGGTTGAGAACGGGAATTG	55.0	50.0	57.7	58.1	794 bp	1.0	77.4
trnR-ACG-trnN-GUU_p5	CAGAGGATTAGAGCACGTGG	ACCTCCTGTTGTTCCTCAGT	55.0	50.0	57.8	58.2	721 bp	1.0	77.2