Hotspot

ycf4-cemA

LSC · 60427-61177 · 751 bp

Back to Poikilospermum

Pi 0.00266

MarkerSeek score 60.30

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 751
variable_sites: 2
pi: 0.00266
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	ATTGAACAGAAAGCCGCTGA	GAGCGATCCACCAAGGTAAA	45.0	50.0	58.1	57.6	884-886 bp	1.0	76.2
ycf4-cemA_p2	TGAACAGAAAGCCGCTGAAT	GAGCGATCCACCAAGGTAAA	45.0	50.0	58.1	57.6	882-884 bp	1.0	76.2
ycf4-cemA_p3	ATTGAACAGAAAGCCGCTGA	AGAGCGATCCACCAAGGTAA	45.0	50.0	58.1	58.4	885-887 bp	1.0	75.9
ycf4-cemA_p4	TGAACAGAAAGCCGCTGAAT	AGAGCGATCCACCAAGGTAA	45.0	50.0	58.1	58.4	883-885 bp	1.0	75.9
ycf4-cemA_p5	TGAACAGAAAGCCGCTGAAT	ATGAGAGAGCGATCCACCAA	45.0	50.0	58.1	58.5	888-890 bp	1.0	75.3