Hotspot

rps16-trnQ-UUG

LSC · 6096-6716 · 621 bp

Back to Poikilospermum

Pi 0.00169

MarkerSeek score 64.20

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 621
variable_sites: 1
pi: 0.00169
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	669-694 bp	1.0	81.8
rps16-trnQ-UUG_p2	GGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.4	58.1	683-708 bp	1.0	79.9
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	GGGTTTTGGTCCCGGTATTC	45.0	55.0	58.1	58.5	690-715 bp	1.0	78.0
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	45.0	50.0	58.1	57.3	673-698 bp	1.0	77.0
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	TAAGGCAACGGGTTTTGGTC	45.0	50.0	58.1	58.7	699-724 bp	1.0	76.8