Hotspot

ycf2

Genome · 47534-54457 · 6924 bp

Pi 0.0319

MarkerSeek score 58.20

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: Genome
length_bp: 6924
variable_sites: 328
pi: 0.0319
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	TCTTGTTTAACGTTTTTGCT	TTTTGGGGTGAAGCTGATCC	30.0	50.0	52.1	58.1	473 bp	0.333333	13.3
ycf2_p2	TCTTGTTTAACGTTTTTGCT	GGGGTGAAGCTGATCCAATT	30.0	50.0	52.1	57.9	469 bp	0.333333	13.3
ycf2_p3	AACGTTTTTGCTATTCAAACT	TTTTGGGGTGAAGCTGATCC	28.6	50.0	52.8	58.1	465 bp	0.333333	13.3
ycf2_p4	ACGTTTTTGCTATTCAAACTT	TTTTGGGGTGAAGCTGATCC	28.6	50.0	52.8	58.1	464 bp	0.333333	13.3
ycf2_p5	ATCTTGTTTAACGTTTTTGCT	TTTTGGGGTGAAGCTGATCC	28.6	50.0	52.8	58.1	474 bp	0.333333	13.3