Hotspot

ycf1

Genome · 107689-112500 · 4812 bp

Pi 0.0325

MarkerSeek score 58.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: Genome
length_bp: 4812
variable_sites: 233
pi: 0.0325
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGTTTGATGGCGCTCTCTTT	ACCACAAGATTGGAAAAATCAGG	45.0	39.1	57.8	57.5	472-478 bp	1.0	59.3
ycf1_p2	AGTTTGATGGCGCTCTCTTT	CCACAAGATTGGAAAAATCAGGT	45.0	39.1	57.8	57.5	471-477 bp	1.0	59.3
ycf1_p3	ACGAATAGTTTGATGGCGCT	ACCACAAGATTGGAAAAATCAGG	45.0	39.1	58.0	57.5	484 bp	0.666667	45.6
ycf1_p4	ACGAATAGTTTGATGGCGCT	CCACAAGATTGGAAAAATCAGGT	45.0	39.1	58.0	57.5	483 bp	0.666667	45.6
ycf1_p5	GACGAATAGTTTGATGGCGC	CCACAAGATTGGAAAAATCAGGT	50.0	39.1	57.6	57.5	484 bp	0.333333	22.6