Hotspot

chlN-trnN-GUU

Genome · 114087-115815 · 1729 bp

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Pi 0.0383
MarkerSeek score 79.40
Primer available yes
Label chlN-trnN-GUU

Feature Metadata

label_name
chlN-trnN-GUU
feature_type
igs
region
Genome
length_bp
1729
variable_sites
96
pi
0.0383
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
chlN-trnN-GUU_p1 TCGCATTCGAAAGTGAGAGT AGGACTTGAACCTACGACCA 45.0 50.0 57.3 58.0 1783-1832 bp 1.0 81.7
chlN-trnN-GUU_p2 TCGCATTCGAAAGTGAGAGT CTCCCCAGGTAGGACTTGAA 45.0 55.0 57.3 58.0 1793-1842 bp 1.0 81.3
chlN-trnN-GUU_p3 TCGCATTCGAAAGTGAGAGT ATCTCCCCAGGTAGGACTTG 45.0 55.0 57.3 57.8 1795-1844 bp 1.0 80.3
chlN-trnN-GUU_p4 TCGCATTCGAAAGTGAGAGTT AGGACTTGAACCTACGACCA 42.9 50.0 58.0 58.0 1783-1832 bp 1.0 79.3
chlN-trnN-GUU_p5 TCGCATTCGAAAGTGAGAGT CCCAGGTAGGACTTGAACCT 45.0 55.0 57.3 58.3 1790-1839 bp 1.0 79.0