Hotspot

rpl32-trnP-GGG

Genome · 99690-99944 · 255 bp

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Pi 0.0801
MarkerSeek score 78.30
Primer available yes
Label rpl32-trnP-GGG

Feature Metadata

label_name
rpl32-trnP-GGG
feature_type
igs
region
Genome
length_bp
255
variable_sites
28
pi
0.0801
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnP-GGG_p1 ACGCCATTATTGAAACCGGA GTGATGGAGATCGTGGGTTC 45.0 55.0 57.9 58.1 889-914 bp 1.0 86.3
rpl32-trnP-GGG_p2 ACGCCATTATTGAAACCGGA ATTTCACCATGGCGGCTTAT 45.0 45.0 57.9 57.9 1049-1074 bp 1.0 86.2
rpl32-trnP-GGG_p3 ACGCCATTATTGAAACCGGA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 57.9 57.9 1072-1097 bp 1.0 85.6
rpl32-trnP-GGG_p4 ACGCCATTATTGAAACCGGA ATGGGGCATAGCGTAGTTTG 45.0 50.0 57.9 58.0 927-951 bp 0.666667 71.1
rpl32-trnP-GGG_p5 ACGCCATTATTGAAACCGGA CTTGGGTGATGGAGATCGTG 45.0 55.0 57.9 58.1 919 bp 0.333333 52.0