Hotspot

trnC-GCA-ycf66

Genome · 80527-81027 · 501 bp

Back to Pohlia

Pi 0.0522
MarkerSeek score 79.10
Primer available yes
Label trnC-GCA-ycf66

Feature Metadata

label_name
trnC-GCA-ycf66
feature_type
igs
region
Genome
length_bp
501
variable_sites
37
pi
0.0522
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
79.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-ycf66_p1 ATCCTTTACCCCCAGTTCGA GGTTGGCGTTTAGATCCCAT 50.0 50.0 58.0 57.9 788-806 bp 1.0 87.9
trnC-GCA-ycf66_p2 AAGTGGTAAGGCAGAGGACT GGTTGGCGTTTAGATCCCAT 50.0 50.0 58.0 57.9 812-830 bp 1.0 87.7
trnC-GCA-ycf66_p3 GCGATATGGCCAAGTGGTAA GGTTGGCGTTTAGATCCCAT 50.0 50.0 58.0 57.9 823-841 bp 1.0 87.5
trnC-GCA-ycf66_p4 CAAGTGGTAAGGCAGAGGAC GGTTGGCGTTTAGATCCCAT 55.0 50.0 57.9 57.9 813-831 bp 1.0 87.1
trnC-GCA-ycf66_p5 AAGTGGTAAGGCAGAGGACT AAGGTTGGCGTTTAGATCCC 50.0 50.0 58.0 57.9 814-832 bp 1.0 87.1