Hotspot

psbI-trnS-GCU

Genome · 62193-62297 · 105 bp

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Pi 0.0635
MarkerSeek score 79.30
Primer available yes
Label psbI-trnS-GCU

Feature Metadata

label_name
psbI-trnS-GCU
feature_type
igs
region
Genome
length_bp
105
variable_sites
10
pi
0.0635
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbI-trnS-GCU_p1 AATGATCCTGGCCGTAATCC GGGTTCGAATCCCTCTCTCT 50.0 55.0 57.4 58.2 161-165 bp 1.0 80.6
psbI-trnS-GCU_p2 AATGATCCTGGCCGTAATCC GAGATGGCCGAGTGGTTTAA 50.0 50.0 57.4 57.6 222-226 bp 1.0 77.3
psbI-trnS-GCU_p3 AATGATCCTGGCCGTAATCC AGAGATGGCCGAGTGGTTTA 50.0 50.0 57.4 58.4 223-227 bp 1.0 77.0
psbI-trnS-GCU_p4 TCAAATGATCCTGGCCGTAA GGGTTCGAATCCCTCTCTCT 45.0 55.0 56.9 58.2 164-168 bp 1.0 76.3
psbI-trnS-GCU_p5 AAATGATCCTGGCCGTAATCC GGGTTCGAATCCCTCTCTCT 47.6 55.0 58.1 58.2 162-166 bp 1.0 76.0