Hotspot

psbK-psbI

Genome · 61582-62081 · 500 bp

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Pi 0.0527
MarkerSeek score 76.80
Primer available yes
Label psbK-psbI

Feature Metadata

label_name
psbK-psbI
feature_type
igs
region
Genome
length_bp
500
variable_sites
39
pi
0.0527
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbK-psbI_p1 TCAAAACCTGCTGCCTTACC GGGTTCGAATCCCTCTCTCT 50.0 55.0 58.4 58.2 1210-1226 bp 1.0 80.4
psbK-psbI_p2 TCAAAACCTGCTGCCTTACC CCCTCTCTCTCCTTCAACCA 50.0 55.0 58.4 58.1 1205-1216 bp 0.666667 66.1
psbK-psbI_p3 TCAAAACCTGCTGCCTTACC GATGGGAATCCGAGAACGAG 50.0 55.0 58.4 57.9 1051 bp 0.333333 49.1
psbK-psbI_p4 TCAAAACCTGCTGCCTTACC GGGAATCCGAGAACGAGAAG 50.0 55.0 58.4 57.8 1048 bp 0.333333 48.6
psbK-psbI_p5 TCAAAACCTGCTGCCTTACC ATGGGAATCCGAGAACGAGA 50.0 50.0 58.4 58.2 1050 bp 0.333333 48.5