Hotspot

trnH-GUG-psbA

Genome · 54887-55017 · 131 bp

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Pi 0.12
MarkerSeek score 78.50
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
Genome
length_bp
131
variable_sites
22
pi
0.12
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
78.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 ACTGCCTTAATCCACTTGGC GTAACGCTCACAACTTCCCT 50.0 50.0 58.2 57.8 223-230 bp 1.0 85.2
trnH-GUG-psbA_p2 CTTAATCCACTTGGCCACGT GTAACGCTCACAACTTCCCT 50.0 50.0 58.2 57.8 218-225 bp 1.0 85.2
trnH-GUG-psbA_p3 TTAATCCACTTGGCCACGTC GTAACGCTCACAACTTCCCT 50.0 50.0 58.2 57.8 217-224 bp 1.0 85.2
trnH-GUG-psbA_p4 GATCCACAATCCACTGCCTT GTAACGCTCACAACTTCCCT 50.0 50.0 57.9 57.8 235-242 bp 1.0 85.0
trnH-GUG-psbA_p5 GATCCACAATCCACTGCCTT TGAACGTAACGCTCACAACT 50.0 45.0 57.9 57.8 240-247 bp 1.0 84.5